However, there were significant differences between the nested PCR and the routine PCR and the nested polA PCR was much more sensitive than the routine polA PCR. 巢式和常规polAPCR结果有显著性差异,巢式PCR敏感性高于常规PCR。
The results showed that PCR with nested primers can detect even 10 molecules of template DNA, but the routine PCR can not detect the template DNA molecules less than 1 000.The results suggested that PCR with nested primers could greatly promote the sensitivity of polymerase chain reaction. 结果表明,当模板DNA分子的数量低于1000个时,常规单对引物PCR反应不能检出,而套式引物PCR反应则可检出低至10个分子的模板DNA。